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. 2019 Sep 11;10:1002. doi: 10.3389/fphar.2019.01002

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Copyright © 2019 Qiu, Wang, Liu, Wang, Zhao, Zhou, Ji, Wang, Xia, Wang, Lin, Liu, Huang and Jiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apigenin induced apoptosis in Eca-109 cell. (A-C) Cells were treated with the indicated concentrations of apigenin, and the apoptosis was detected by FCM annexin V/PI staining. The protein expression levels were examined by Western blot, and GAPDH was used as loading control. (D–F) Cells were pretreated with 50 ng/µl IL-6, prior to the addition of indicated concentrations of apigenin, and the apoptosis was detected by FCM annexin V/PI staining. The protein expression was examined by Western blot. (A, D) the representative charts, (B, E) the quantified data, and (C, F) the Western blot results. *P < 0.05 and **P < 0.01 indicate significant difference as compared to the corresponding control (n = 3).