Figure S6. Localization of NLS-td-Tomato-NES in WT and TDP-43 KO cells is unaltered.

(A) Representative confocal images showing nuclear localization of the NLS-td-Tomato reporter in both WT and KO cell lines. Nuclei were identified with DAPI staining. (B) Nuclear/cytoplasmic fluorescence ratio was measured using Cell Profiler (n = 3 biological replicates in which a total of 686 WT and 469 KO cells were analyzed; P = 0.8; error bars show mean ± SEM). Scale bar = 10 μm.