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. 2019 Sep 16;2(5):e201900489. doi: 10.26508/lsa.201900489

Figure 5. Hsp104 is found in mHtt particles and is required for their formation.

Figure 5.

(A) Representative images of mHtt(72Q)-GFP in wild-type (left panel) and hsp104Δ (right panel) cells. Bar, 4 μm. (B) Cells containing genomically tagged Hsp104-mCherry and either native Htt(25Q)-GFP (upper panels) or mHtt(72Q)-GFP (lower panels). Hsp104-mCherry is displayed as magenta. Arrow, mHtt IB containing Hsp104; arrowheads, Hsp104 IB with no detectable mHtt. Bar, 1 μm. (C, D) Cells from the strains shown in (B) were assessed for the presence of IBs and small particles. The fraction of cells containing one or more IBs (C) or small particles (D) bearing the indicated marker was determined. **P = 0.0001–0.0003 for differences between Htt alleles, two-way ANOVA, n = 4 replicates, 124–130 cells total per group; whiskers indicate minimum and maximum values.