Figure 6. Mutant Htt IBs are non–membrane bound.

Transmitted electron micrograph of a cell expressing mutant Htt(72Q)-GFP, fixed, and stained with anti-GFP and 10-nm gold particle–conjugated secondary antibody. Dotted line indicates a cluster of gold particles, ∼500 nm in diameter, in the cytoplasm. V, a lobe of the vacuole, surrounded by membrane. Bar, 500 nm.