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. 2019 Sep 16;2(5):e201900489. doi: 10.26508/lsa.201900489

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© 2019 Aktar et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S7. — (A) Mutant Htt(72Q)-GFP (green) co-expressed in cells with an integrated Spc42-mCherry reporter fusion (magenta). The GFP-tagged IBs and mCherry-tagged spindle poles are visible in different planes. The merged image demonstrates the lack of co-localization in the x–y plane; the magenta and green images are also 1.6 μm apart along the z-axis. (B) Mutant Htt(103Q)-GFP (green) co-expressed in cells with an integrated Spc42-mCherry reporter fusion (magenta). The magenta image is a maximum projection as the two mCherry-tagged spindle poles were visible in planes that were 600 nm apart; in this case, the IB, which is much larger than a spindle pole, was visible in the same planes as the two spindle poles. (C) Mutant Htt(72Q)-GFP (green) co-expressed in cells with an integrated Spc72-mCherry reporter fusion (magenta). The magenta image is a maximum projection as the two mCherry-tagged spindle poles were visible in planes that were 300 nm apart; in this case, the IB was visible in the same planes as the two spindle poles. The scale bar represents 2 μm in (A–C).