Immunohistochemical staining for SDF-1. (A) Malignant cells exhibit absent SDF-1 staining in MK- group, magnification 200×; (B) malignant cells exhibit (+) SDF-1 staining in MK- group, magnification 400×; (C) malignant cells exhibit (++) SDF-1 staining in MK+ group, magnification 200×; (D) malignant cells exhibit (+++) SDF-1 staining in MK+ group, magnification 400×. SDF-1, stromal cell-derived factor-1; MK, megakaryocyte.