Table 1.
Characterization and content (mg/g dry matter (d.m.)) of main compounds of Blue Congo extract. Rt—retention time.
| Peak No. | Compound | Rt (min) | [M − H]+/[M + H]− (m/z) | MS/MS Fragments (m/z) | Content (mg/g d.m.) |
|---|---|---|---|---|---|
| Anthocyanins | |||||
| 1 | Petunidin-3-O-rutinoside-5-O-glucoside | 3.52 | 787.2288+ | 479.1120/317.0676 | 4.42 ± 0.23 |
| 2 | Petunidin-3-O-p-caffeyl-rutinoside-5-O-glucoside | 6.50 | 949.2610+ | 787.2059/479.1165/317.0676 | 7.41 ± 0.33 |
| 3 | Delphinidin-3-O-p-coumaroyl-rutinoside-5-O-glucoside | 6.68 | 919.2560+ | 757.2056/465.1026/303.0494 | 2.79 ± 0.24 |
| 4 | Petunidin-3-O-p-coumaryl-rutinoside-5-O-glucoside | 7.19 | 933.2700+ | 771.2118/479.1210/317.0676 | 53.95 ± 2.55 |
| 5 | Malwidin-3-O-p-coumaryl-rutinoside-5-O-glucoside | 7.69 | 947.2831+ | 785.2213/493.1294/131.0825 | 4.10 ± 0.19 |
| Phenolic Acid | |||||
| 1 | 3-O-Caffeoylquinic acid (neochlorogenic acid) | 3.38 | 353.0338− | 191.0562/179.0342 | 24.22 ± 1.03 |
| 2 | 4-O-Caffeoylquinic acid (cryptochlorogenic acid) | 4.59 | 353.0915− | 173.0458/179.0342/191.0534 | 29.69 ± 1.33 |
| 3 | Methyl-3-caffeoylquinate | 4.70 | 367.1057− | 161.0237 | 1.81 ± 0.11 |
| 4 | 5-O-Caffeoylquinic acid (chlorogenic acid) | 4.85 | 353.0838− | 707.1813/191.0563 | 98.65 ± 4.74 |
| 5 | Methyl-4-caffeoylquinate | 5.63 | 367.1051− | 161.0237 | 6.64 ± 0.26 |
| 6 | Methyl-5-caffeoylquinate | 6.41 | 367.1057− | 179.0342/161.0237/135.0450 | 6.39 ± 0.31 |
| Total | Anthocyanins and phenolic acid | 237.07 ± 11.32 | |||
Data are expressed as means ± SD, n = 3. Anthocyanins are expressed as petunidin-3-O-glucoside equivalents; phenolic acids are expressed as chlorogenic acid equivalents.