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. 2019 Aug 25;24(17):3084. doi: 10.3390/molecules24173084

Figure 5.

Figure 5

Effects of melatonin and EX527 on apoptosis of mouse Leydig cells. (A) The effects of melatonin and EX527 on apoptosis rate of mouse Leydig cells (n = 3). The four quadrants in the figure represent dead cells (Q2-1), late-stage apoptotic cells (Q2-2), viable cells (Q2-3), and early-stage apoptotic cells (Q2-4). The apoptosis rate is the sum of values from Q2-2 and Q2-4. The relative mRNA expression levels of BAX (B) and BCL-2 (C) (n = 3). (D) The relative protein expression levels of SIRT1, BAX, and BCL-2 were detected and analyzed (n = 3). Values are shown as mean ± SEM. ** p < 0.01 or * p < 0.05 compared with the control group; ### p < 0.001, ## p < 0.01 or # p < 0.05 compared with melatonin-only treatment group.