Table 4.
Analysis of variance (ANOVA) for the olive pomace extracts obtained by PLE.
| Source | Sum of Squares | d.f. | Mean Square | F-Ratio | p-Value | R2 | R2 adj. d.f. |
|---|---|---|---|---|---|---|---|
| Hydroxytyrosol | |||||||
| A: Temperature | 42324.8 | 1 | 42324.8 | 42324.85 | 0.0000∗ | 88.473 | 78.8671 |
| B: Ethanol | 1623.56 | 1 | 1623.56 | 1623.56 | 0.0000∗ | ||
| AA | 14278.4 | 1 | 14278.4 | 14278.42 | 0.0000∗ | ||
| AB | 6511.91 | 1 | 6511.91 | 6511.91 | 0.0000∗ | ||
| BB | 409.411 | 1 | 409.411 | 409.41 | 0.0000∗ | ||
| Lack-of-fit | 9738.63 | 3 | 3246.21 | 3246.21 | 0.0000* | ||
| Pure error | 94.9681 | 3 | 31.656 | ||||
| Total (corr.) | 82912.1 | 11 | |||||
| Hydroxy D-oleuropein aglycone | |||||||
| A: Temperature | 118.618 | 1 | 118.618 | 0.36 | 0.5704 | 86.4431 | 75.1457 |
| B: Ethanol | 523.819 | 1 | 523.819 | 1.59 | 0.2541 | ||
| AA | 10127.7 | 1 | 10127.7 | 30.75 | 0.0015∗ | ||
| AB | 25.1495 | 1 | 25.1495 | 0.08 | 0.7916 | ||
| BB | 1619.86 | 1 | 1619.86 | 4.92 | 0.0479* | ||
| Lack-of-fit | 1016.74 | 3 | 338.913 | 1.64 | 0.3481 | ||
| Pure error | 1976.45 | 6 | 329.408 | ||||
| Total (corr.) | 14578.9 | 11 | |||||
| Hydroxy-oleuropein | |||||||
| A: Temperature | 3656.18 | 1 | 3656.18 | 3,.6 | 0.1065 | 80.7716 | 64.748 |
| B: Ethanol | 2401.14 | 1 | 2401.14 | 2.37 | 0.175 | ||
| AA | 17567.8 | 1 | 17567.8 | 17.31 | 0.0059∗ | ||
| AB | 2.34565 | 1 | 2.34565 | 0 | 0.9632 | ||
| BB | 3117.69 | 1 | 3117.69 | 3.07 | 0.1302 | ||
| Lack-of-fit | 4635.83 | 3 | 1545.28 | 6.46 | 0.0798 | ||
| Pure error | 6090.85 | 6 | 1015.14 | ||||
| Total (corr.) | 31676.3 | 11 | |||||
| Demethyl oleuropein | |||||||
| A: Temperature | 69.2627 | 1 | 69.2627 | 3.98 | 0.0929 | 89.4383 | 80.6369 |
| B: Ethanol | 239.089 | 1 | 239.089 | 13.76 | 0.0100∗ | ||
| AA | 461.585 | 1 | 461.585 | 26.56 | 0.0021∗ | ||
| AB | 109.428 | 1 | 109.428 | 6.3 | 0.0460∗ | ||
| BB | 6.55101 | 1 | 6.55101 | 0.38 | 0.5618 | ||
| Lack-of-fit | 52.3368 | 3 | 17.4456 | 1.01 | 0.4976 | ||
| Pure error | 104.287 | 6 | 17.3811 | ||||
| Total (corr.) | 987.409 | 11 | |||||
| Decarboxymethyl oleuropein aglycone | |||||||
| A: Temperature | 14.449 | 1 | 14.449 | 1.61 | 0.251 | 91.0723 | 83.6326 |
| B: Ethanol | 13.6533 | 1 | 13.6533 | 1.52 | 0.263 | ||
| AA | 425.539 | 1 | 425.539 | 47.53 | 0.0005∗ | ||
| AB | 7.32752 | 1 | 7.32752 | 0.82 | 0.4005 | ||
| BB | 81.121 | 1 | 81.121 | 9.06 | 0.0237∗ | ||
| Lack-of-fit | 7.96575 | 3 | 2.65525 | 0.25 | 0.8548 | ||
| Pure error | 53.7187 | 6 | 8.95311 | ||||
| Total (corr.) | 601.709 | 11 | |||||
| Oleuropein | |||||||
| A: Temperature | 5650.06 | 1 | 5650.06 | 38.15 | 0.0008∗ | 93.7987 | 88.631 |
| B: Ethanol | 1826.83 | 1 | 1826.83 | 12.34 | 0.0126∗ | ||
| AA | 3976.57 | 1 | 3976.57 | 26.85 | 0.0021∗ | ||
| AB | 2987.01 | 1 | 2987.01 | 20.17 | 0.0041∗ | ||
| BB | 76.7892 | 1 | 76.7892 | 0.52 | 0.4985 | ||
| Lack-of-fit | 329.614 | 3 | 109.871 | 0.59 | 0.6639 | ||
| Pure error | 888.52 | 6 | 148.087 | ||||
| Total (corr.) | 14328 | 11 | |||||
| Yield | |||||||
| A: Temperature | 295.563 | 1 | 295.563 | 108.09 | 0.0000∗ | 96.5258 | 93.6305 |
| B: Ethanol | 23.3825 | 1 | 23.3825 | 8.55 | 0.0265∗ | ||
| AA | 27.7676 | 1 | 27.7676 | 10.15 | 0.0189∗ | ||
| AB | 37.7949 | 1 | 37.7949 | 13.82 | 0.0099∗ | ||
| BB | 36.3875 | 1 | 36.3875 | 13.31 | 0.0107∗ | ||
| Lack-of-fit | 10.3376 | 3 | 3.44588 | 1.83 | 0.3165 | ||
| Pure error | 16.4071 | 6 | 2.73452 | ||||
| Total (corr.) | 472.249 | 11 | |||||
d.f.: degrees of freedom. ∗ Statistically significant.