Table 1.
Bulk separation results from the silica column, based on polarity and affinity of terpenes and phytocannabinoids extracted from two commercial strains of cannabis: Cs12 (chemotype I) and Arbel (chemotype III). Hex: n-hexane; EtAc: ethyl acetate; EtOH: ethanol; CBL: cannabicyclol; CBC: cannabichromene; CBD: cannabidiol; THC: tetrahydrocannabinol; CBG: cannabigerol.
| Fraction No. | Solvent | Compounds in Fractions and Their Relative Abundances | |||||
|---|---|---|---|---|---|---|---|
| A. Cs12 | B. Arbel | ||||||
| Amount (mg) | Compound i.d. | % | Amount (mg) | Compound i.d. | % | ||
| 1 | Hex | 0.3 | 2 | alkanes | |||
| 2 | Hex | 0.1 | 18.4 | α-Pinene Camphene β-Pinene alkanes |
23 1 11 64 |
||
| 3 | Hex | 3.7 | alkanes | 6.2 | α-Pinene β-Pinene D-Limonene trans-α-Bergamotene Aromadendrene γ-Selinene Guaiadiene δ-Selinene Selinadiene β-Maaliene |
4 6 38 12 20 3 3 6 4 2 |
|
| 4 | Hex:EtAc 7:3 | 11.1 | alkanes | 0.6 | |||
| 5 | Hex:EtAc 1:1 | 0.3 | α-Pinene Camphene β-Pinene (+)3-Carene |
36 2 43 19 |
47 | Linalool Fenchol Terpinolene β-Famesene Humulne α-Famesene cis-α-Bergamotene Guaiol γ-Eudesmol α-Eudesmol α-Bisabolol phytol CBL CBC CBD δ8-THC δ9-THC CBG γ-Sitosterol |
2 3 1 2 1 1 1 1 2 3 1 1 1 1 5 42 8 14 5 |
| 6 | Hex:EtAc 3:7 | 73.2 | β-Thujene α-Pinene Camphene β-Pinene α-Phellandrene α-Terpinene β-Phellandrene isosativene α-Guaiene |
3 2 1 23 9 15 23 15 9 |
66 | Linalool Fenchol Terpinolene Guaiol β-Selinene γ-Eudesmol α-Eudesmol phytol CBC CBD δ8-THC δ9-THC CBG |
1 2 1 5 1 2 2 4 9 53 2 13 3 |
| 7 | EtAc | 3 | β-Pinene α-Phellandrene α-Terpinene β-Phellandrene cis-α-Bergamotene isosativene trans-α-Bergamotene α-Guaiene α-Curcumene |
1 8 1 18 3 3 23 43 1 |
22 | γ-Eudesmol α-Eudesmol CBL CBC CBD δ8-THC δ9-THC CBG ketone |
5 2 1 4 53 9 2 13 3 |
| 8 | EtOH | 57.6 | β-Myrcene α-Phellandrene 2-Carene D-Limonene β-Ocimene γ-Terpinolene 3-Carene Citral m-Cymenol cis-α-Bergamotene Caryophyllene trans-α-Bergamotene α-Guaiene β-Famesene Humulne Giuaia-9,11-diene α-Bulnesene β-phellandrene α-Bisabolene Nerolidol α-Bisabolol phytol CBC CBD THC CBG |
4 1 3 4 1 12 1 1 1 9 1 4 3 1 5 4 1 1 2 1 1 1 1 3 31 3 |
0.1 | Linalool oxide Limonene oxide hydroxylinalool γ-Eudesmol α-Eudesmol alkanes CBL CBC CBD δ8-THC δ9-THC CBG |
1 0.3 1 2 2 4 6 1 60 2 15 4 |