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(A) HCP hydrogel formation. Carboxyl groups in HA or CS were reacted with cystamine dihydrochloride by activation with EDC and NHS. Reduction of cystamine by DTT resulted in free thiol groups in the HA or CS backbone. Cross-linking of HA-SH and CS-SH mixtures by conjugate addition using PEGDA. 1H NMR spectra confirmed successful synthesis of (B) HA-SH and (C) CS-SH.
