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. Author manuscript; available in PMC: 2020 Feb 5.
Published in final edited form as: J Appl Polym Sci. 2018 Nov 1;136(5):47212. doi: 10.1002/app.47212

Figure 6.

Figure 6.

Dynamic FAK phosphorylation is dependent on hydrogel gelation during encapsulation. (A) Schematic of monitoring FAK phosphorylation of live hMSCs containing FAKSOR within hydrogels by FLIM. (B) Fluorescence lifetime images of hMSCs encapsulated in HCP3400 hydrogels as a function of time. Scale bar 20 μm. (C) Quantitative analysis showed a single hMSC gradually increased FAK phosphorylation over time during cell encapsulation in HCP3400 hydrogels(n=3). (D) FAKSOR average lifetime in hMSC-laden HCP3400 hydrogels remained constant over 30 minutes right after G’ reached plateau (n=5). All data are reported as mean ± SD.