Fig. 4.

Abscopal anti-tumor efficacy of intratumoral STING activation in combination with systemic checkpoint antibodies. Mice were inoculated with mEER tumor cells both in the flank (1 × 10⁶) and tongue (4 × 10⁴) and treated with intratumoral (i.t.) administration of STING agonist (ML-RR CDA) on days 10 and 16 along with or without immunotherapy employing individual or combinations of α-PD-1 and α-CTLA-4 antibodies on days 10, 13, 16, and 19 (a). Growth of flank-implanted tumors over time for individual mice in different treatment groups is expressed in terms of tumor area (mm²) in (b). The data is pooled from three separate experiments and the total number of mice in each group is noted. The survival curves for mice in different treatment groups are shown in (c). Statistical significance for differences in survival of mice in different combination treatment groups relative to untreated control group were calculated using Log-rank (Mantel-Cox) test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001