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Representative chromatograms of standard solution and dog saliva sample. Blue line represent OT (1007.2 → 723.2 m/z), red line represent OT-d5 (1012.2 → 723.2 m/z). OT standard solution contained 500 pg/mL OT. A 300 µL of OT standard solution or dog saliva was extracted with 1.2 mL 80% aqueous ACN containing 1000 pg/mL OT-d5.
