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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Methods Mol Biol. 2019;1955:135–146. doi: 10.1007/978-1-4939-9148-8_10

Fig. 2.

Fig. 2

Kinetics of SA incorporation onto the surface of T. cruzi trypomastigotes. Flow cytometry-based determination of Neu5Az transfer to live cell-derived trypomastigotes using Neu5Azα2–3LacβOMe compound as SA donor. The amount of Neu5Az transferred was revealed by dibenzyl cyclooctene-FLAG and anti-FLAG antibody. (a) Typical cytogram obtained at the end of the Neu5Az transfer protocol. (b) Correlation between the concentration of Neu5Azα2–3LacβOMe compound used in the assay and the amount of cell-derived trypomastigote labeling