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Features and recommended applications of different bioorthogonal probes
| Copper-catalyzed reaction | Copper-free reactions | |
|---|---|---|
| Azide-reactive compounds structure |
 Alkyne |
 Phosphine |
 Cyclooctyne |
||
| Suppliers | Thermo-Fisher | Thermo-Fischer |
| Jena Bioscience | Cayman Chemical | |
| Click Chemistry Tools | Jena Bioscience | |
| Click Chemistry Tools | ||
| Western blot | ✓ | ✓ |
| Immunoprecipitation | ✓ | |
| Flow cytometry/immunofluorescence (fixed cells) | ✓ | ✓ |
| Flow cytometry/immunofluorescence (live cells) | ✓ |
