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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Anal Chem. 2016 Oct 14;88(21):10404–10410. doi: 10.1021/acs.analchem.6b01961

Figure 2.

Figure 2.

Functional characteristics of the binary reagent system. The LCST of mNPs was 36-37°C and salt-controllable (A). The mNPs can be separated with ~97% efficiency in less than 1 minute (n=2) after a temperature stimulus of 37°C (B). Standard deviations were <1.5% at each data point. SEC chromatograms shows high molecular weight polymer-Ab conjugates resolved from unreacted polymer and Ab. (C) Polymer-Ab conjugates (conj) were only captured in the presence of mNPs, and there is no non-specific capture of the native Ab (IgG) by mNPs (D).