Table 1.

Biophysical and kinetic properties of unconjugated anti-p24 antibody (Ab), stimuli-responsive polymer-Ab conjugates and antibody-labeled Dynabeads® measured by SPR, ELISA and binding progress analyses.

Method	Sample	Kd (nM)	kon (M−1s−1)	koff (s−1)	τ1/2 (h)	Rmax (R.U.) Fitted	Rmax (R.U.) Theoretical	$\frac{\mathit{R}\mathit{m}\mathit{a}\mathit{x}\text{Fit}}{\mathit{R}\mathit{m}\mathit{a}\mathit{x}\text{Theor}}\times 100$
SPR	Ab	0.165	2.42×l05	4.00×10−5	4.8	11.6	24.0	48.3 %
	Polymer-Ab	0.501	2.06×l05	10.3×l0−5	1.9	7.6	21.7	35.1%
ELISA	Ab	1.25	-	-	-	-	-	-
	Polymer-Ab	2.27	-	-	-	-	-	-
	Dynabeads®-anti-p24	2.45	-	-	-	-	-	-
Binding Progress	Polymer-Ab	1.7	3.6×105	61.4×l0−5	0.32	-	-	-
	Dynabeads®-anti-p24	2.4	0.37×l05	8.8×l0−5	2.2	-	-	-