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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Ann Plast Surg. 2019 Oct;83(4):404–410. doi: 10.1097/SAP.0000000000001996

Figure 4. Effect of Wound Solutions on C. albicans.

Figure 4.

A-C. Biofilms were formed in 100 μl RPMI with MOPS. C. albicans ATCC MYA-2876/SCS314 (panels A-C) or clinical strains of C. albicans (panel D). Organisms were added to tissue culture treated plates and incubated for 24 h at 35°C. After 24 h biofilms were washed with 1X PBS three times and 100 μl 100%, 50% or 25% Vashe (panel A), PhaseOne (panel B) or Mafenide (panel C) were added to the wells. Control wells contained 100 μl 1X PBS. Plates were incubated for 1 m, 10 m, 60 m or 24 h. Wells were washed three times with PBS to remove the wound solutions and reconstituted with 100 μl H2O. Bacterial viability was monitored by the CFU assay. D. This data represents the minimum time to kill (CFU=0) clinical strains of C. albicans with Vashe, PhaseOne or Mafenide. Yeast cells that were not killed at 24 hare indicated by >24 h.

Panels A-D are representative of 3 independent experiments.