Fig. 1.

VMAT2: [³H]DA uptake in subcellular fractions: synaptosomal (A), membrane-associated (B), and vesicular (C). Taar1 WT and KO mice received four intraperitoneal injections of saline or MA (5 mg/kg), 2 hours apart, and were euthanized 24 hours following the final injection. Striatal tissue from four to five mice per genotype per treatment group was pooled and values normalized to the amount of protein in each sample. Experiments were conducted as described in the text. Data represent means ± S.E.M. of five to seven independent experiments, expressed as percentage of the specific uptake in Taar1 WT control (saline) pooled tissue in each experiment. Mean ± S.E.M. of Taar1 WT control group: synaptosomal, 13.4 ± 3.1 pmol/mg protein; membrane-associated, 13.9 ± 3.5 pmol/mg protein; vesicular, 72.9 ± 21.6 pmol/mg. *P < 0.0001 compared with saline-treated controls; ⁺P < 0.05 between genotypes; ^#P < 0.0001 for the main effect of treatment; ^†P < 0.01 for the main effect of genotype.