Fig. 3.

NCLS treatment attenuates surgery-induced mechanical hypersensitivity and blocks hyperalgesic priming in male and female mice: (A–C). NCLS treatment starting immediately after baseline measurements dose dependently decreased mechanical hypersensitivity after plantar incision surgery and prevented plantar incision-induced hyperalgesic priming precipitated by PGE₂ injection at all doses. *P < 0.05; **P < 0.01; ***P < 0.001; n = 4 for the NCLS groups, n = 3 for the vehicle group. (D–F) NCLS treatment also decreased mechanical hypersensitivity after plantar incision surgery and prevented surgery-induced hyperalgesic priming precipitated by PGE₂ injection. *P < 0.05; **P < 0.01; ***P < 0.001; n = 6 for the NCLS group, n = 6 for the vehicle group.