Figure 2.

Functional complementation assay. (a) Functional complementation of the panD E. coli mutant, which is auxotrophic for β‐alanine. (b) Functional complementation of the E. coli HYE032 mutant, which is auxotrophic for L‐alanine. The plasmids used were pBAD33 and pBAD33::At3g08860. Transformants harboring pBAD33 or pBAD33::At3g08860 were grown to an OD of 1.0 measured at 600 nm. The strains were serially diluted to 10^–1, 10^–2, 10^–3, and 10^–4 using 0.85% (w/v) saline. Five µl was replica plated on M9 medium with or without β‐alanine or L‐alanine supplemented with 0.2% (w/v) arabinose or glucose