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. 2019 Sep 18;3(9):e00171. doi: 10.1002/pld3.171

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© 2019 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Protein purification and biochemical characterization. (a) Purification of the At3g08860 protein to apparent homogeneity by affinity chromatography followed by SDS‐PAGE. Along with the molecular mass markers (kDa) (lane 1), the gel shows the profile of 10 μg of uninduced soluble proteins (lane 2), 10 μg of induced soluble proteins (lane 3) and 0.5 μg of the purified recombinant enzyme (black arrow) (lane 4). (b) A wavelength scan of the protein shows a peak around 420 nm (indicated by the yellow arrow), which is characteristic of PLP‐containing proteins