Skip to main content
. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Ann Integr Mol Med. 2019;1(1):1002.

Figure 1: Projected steps in RdRp-facilitated and antisense RNA-mediated amplification of mammalian mRNA with chimeric RNA end product retaining the intact coding capacity of conventional mRNA.

Figure 1:

Top panel: conventional, genome-originated mRNA molecule. Bottom panel: projected stages of antisense RNA-mediated mRNA amplification. Boxed line – sense strand RNA. Single line – antisense strand RNA. “AUG” – functional translation initiation codon (could be other than “AUG”). “TCE”– 3’-terminal complementary element; “ICE”– internal complementary element, both on the antisense RNA strand. Yellow circle – helicase/modifying activity complex. Blue lines (both single and boxed) – RNA strand modified and separated from its complement by a helicase complex. Red arrowhead – position of cleavage of the chimeric intermediate. Step 1 : synthesis of antisense strand; step 2: strand separation; step 3: folding of antisense strand into self-priming configuration; step 4: extension of self-primed antisense RNA; step 5: strand separation; step 6: cleavage of the chimeric intermediate; stage 7: end-products of amplification. Note that chimeric RNA end product retains the intact coding capacity of conventional mRNA.