Skip to main content
. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Ann Integr Mol Med. 2019;1(1):1002.

Figure 13: Novel experimental design 2: in vivo generation of a chimeric polypeptide containing murine beta globin chain, initiated from the antisense RNA and non-contiguously encoded in the genome.

Figure 13:

Uppercase letters: sense strand RNA. Lowercase letters: antisense strand RNA. Highlighted in green: AUG translation initiation codon. 5’-CAU-3’ (highlighted in blue) on the sense RNA: complement of 5’-aug-3’ (highlighted in green) on the antisense RNA. “INS” highlighted in grey: insert encoding a Tag peptide and lacking initiation codon; “TAG” highlighted in grey: peptide encoded by “INS”. “ins” highlighted in grey: “INS” complement in the antisense RNA. Amino acid sequence highlighted in blue: polypeptide encoded by conventional mRNA or by the amplified mRNA. Amino acid sequence highlighted in grey and underlined: the N-end extension encoded by the antisense RNA. Highlighted in yellow: editing changes resulting in replacement of the “aug” by the “aca” in the antisense RNA. Red arrow: position of cleavage of the chimeric inter mediate. Top panel: A-conventional mRNA encoding beta globin chain. B-chimeric RNA end product of amplification (adopted from Figure 4) encoding the same polypeptide as “A”. C-amino acid sequence encoded by either “A” or “B”. Middle panel: A-projected edited “dormant” mRNA originated from edited beta globin gene. B-antisense complement of edited beta globin mRNA. C-folding of the antisense strand into self-priming configuration; 3’terminal “c” is a transcript of the 5’capG of mRNA. D-extension of self-primed antisense strand into sense-oriented sequence followed by strand separation and cleavage of the chimeric intermediate. E-chimeric RNA end product of RNA-dependent amplification of edited beta globin mRNA. F-projected translational outcome. Bottom panel: same as the middle panel with the exception of editing changes resulting in replacement of the “aug” by the “aca” (highlighted in yellow) on the antisense RNA.