(A) Control miRNA or miR-216a was injected and electroporated into the left eyes of wild-type zebrafish before intense light exposure (0 h). After 45 h, retinas were collected, sectioned, and immunostained using antibodies against β-catenin (red) and PCNA (green). Nuclei were counterstained with TOPRO (blue).
(B) β-Catenin colocalized with PCNA+ proliferating MG after 45 h of intense light damage. β-Catenin accumulation was not detected in miR-216a-over-expressing retinas. Scale bar, 50 µm.