KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-PCNA monoclonal antibody | Sigma | P8825; RRID: AB_477413 |
| Rabbit anti-PCNA polyclonal antibody | Abcam | 18197; RRID: AB_444313 |
| Rabbit anti-GFP polyclonal antiserum | Torrey Pines Biolabs | TP401; RRID: AB_10013661 |
| Mouse anti-β-catenin antibody | BD Bioscience | 610153; RRID: AB_397554 |
| Rabbit anti-dot1l polyclonal antibody | Bethyl labs | A300-954A; RRID: AB_2261948 |
| Donkey anti-mouse AF488 | Jackson Immuno | 715-545-150 |
| Donkey anti-mouse AF647 | Jackson Immuno | 715-605-150 |
| Donkey anti-mouse Cy-3 | Jackson Immuno | 715-165-150 |
| Donkey anti-rabbit Cy3 | Jackson Immuno | 711-165-152 |
| Donkey anti-rabbit AF488 | Jackson Immuno | 711-545-152 |
| Rabbit anti-KMT4/Dot1l polyclonal antibody | Abcam | Ab228766 |
| Rabbit anti-alpha tubulin antibody | Abcam | Ab15246; RRID: AB_301787 |
| Anti-rabbit HRP-conjugated antibody | Cell Signaling Technologies | 7074S;RRID: AB_2099233 |
|
Chemicals, Peptides, and Recombinant Proteins | ||
| Leibovitz L-15 media | ThermoFisher | 21083-027 |
| Hyaluronidase | Sigma-Aldrich | H3884 |
| Papain dissociation kit | Worthington Biochemical Corp | LK003178 |
| DNase I | NEB | M0303S |
| Leupeptin trifluoroacetate salt | Sigma-Aldrich | L2023-10MG |
| GSK-3β Inhibitor I | Calbiochem | 361540 |
| Tankyrase inhibitor/axin stabilizing agen XAV939 | Cayman chemical | 13596 |
| Dot1 inhibitor EPZ004777 | Calbiochem | 532282 |
| TO-PRO-3 Iodide | Invitrogen | T3605 |
| Vectashield | Vector Labs | H-1000 |
|
Critical Commercial Assays | ||
| TRIzol-LS | ThermoFisher | 10296028 |
| Taqman probe: U6 snRNA | Life Technologies | 001973 |
| Taqman probe: hsa-miR-216a | Life Technologies | 002220 |
| TURBO DNAfree kit | ThermoFisher | AM1907 |
| Maxima first strand cDNA synthesis kit | Thermo Scientific | K1641 |
| SsoAdvanced Universal SYBR® Green Supermix | Bio-Rad | 1725271 |
| QuikChange Lightning Site-Directed Mutagenesis Kit | Stratagene | 210518 |
| mMESSAGE mMACHINE® SP6 Transcription Kit | Life Technologies | AM1340 |
| NucAway™ Spin Columns | Life Technologies | AM10070 |
|
Experimental Models: Organisms/Strains | ||
| Zebrafish: Wild-type (AB) | Walker, 1999 | N/A |
| Zebrafish: Tg(1016tuba1a:gfp) | Fausett and Goldman, 2006 | N/A |
| Zebrafish: Tg(gfap:gfp)mi2001 | Bernardos and Raymond, 2006 | N/A |
|
Oligonucleotides | ||
| See Table S1 for oligonucleotides used. | N/A | N/A |
|
Recombinant DNA | ||
| PCS2+ vector | Turner and Weintraub, 1994 | N/A |
|
Software and Algorithms | ||
| ImageJ software 4.13 | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |