| Antibodies |
|
|
| ZV-48 |
Diamond laboratory |
Zhao et al., 2016 |
| MAR1-5A3 (anti-IFNAR1) |
Leinco |
Cat # I-401 |
| WNV E60 (anti-E, fusion loop specific) |
Diamond Laboratory |
NA |
| FITC anti-KLRG1 |
BioLegend |
Cat # 138410 |
| Alexa Fluor 700 anti-CD127 |
eBioSceince |
Cat # 14-1278-82 |
| Alexa Fluor 647 anti-IFNγ |
BD BioScience |
Cat # 557735 |
| Anti-mouse CD16/32 |
eBioSceince |
Cat # 14-0161-85 |
| Fixable Viability Dye eFluor 506 |
Invitrogen |
Cat # 65-0866-14 |
| BUV395 anti-CD45 |
BD BioSciences |
Cat # 564279 |
| Pacific blue anti-CD62L |
BioLegend |
Cat # 104424 |
| PE anti-CD44 |
BioLegend |
Cat # 103024 |
| PE/Cy7 anti-mouse anti-CD4 |
BioLegend |
Cat # 100422 |
| PerCP/Cy5.5 anti-CD8b |
BioLegend |
Cat # 126610 |
| APC/Cy7 anti-CD19 |
BioLegend |
Cat # 115530 |
| Alexa Fluor 488 anti-CD3 |
BioLegend |
Cat # 100210 |
| Fixable Aqua dead cell stain |
Invitrogen |
Cat # L34965
|
| BV 605 anti-TNFα |
BioLegend |
Cat # 506329 |
| anti-CD8α |
BioXcell |
Cat # BE0117 |
| Rat IgG2b isotype control |
BioXcell |
Cat # BE0090 |
| HRP-conjugated goat anti-mouse IgG |
Sigma Aldrich |
Cat # A0168-1ML |
| Anti-Mouse IgG (γ-chain specific)–Biotin antibody |
Sigma Alrich |
Cat# B7022 |
| PE anti-CD138 |
BD BioScience |
Cat # 553714 |
| Anti-PE microbeads |
Miltenyi Biotec |
Cat # 130-048-801 |
| Bacterial and Virus Strains |
|
|
| Zika virus Dakar clone 41525-mouse adapted |
Diamond laboratory |
Gorman et al., 2018 |
| ZIKV H/PF/2013 |
X. de Lamballerie laboratory |
Baronti et al., 2014 |
| GAd-control (GC46Null) |
Precigen |
NA |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Recombinant ZIKV E protein |
Meridian Life Science |
Cat # R01635
|
| ZIKV-derived, Db-restricted peptide E294-302 |
WU Center for Human Immunology |
Elong Ngono et al., 2017 |
| Recombinant ZIKV NS1 protein |
Native Antigen |
Cat # ZIKVSU-NS1 |
| Critical Commercial Assays |
|
|
| RNeasy mini kit |
QIAGEN |
74104 |
| QIAamp viral RNA Mini kit |
QIAGEN |
52906 |
| Taqman RNA-to-CT 1-step kit |
Applied Biosystem |
4392938 |
| Fixation/Permeabilization solution kit |
BD BioSciences |
Cat # 554714 |
| Mitomycin C from Streptomyces caespitosus |
Sigma Aldrich |
Cat # M4287 |
| NP 40 Substitute |
Sigma Aldrich |
Cat # 74385 |
| CD19 microbeads |
Miltenyi Biotec |
Cat # 130-052-201 |
| 1 step Fix/Lyse Solution |
eBioSceince |
Cat # 00-5333-54 |
| Experimental Models: Cell Lines |
|
|
| Vero |
ATCC |
CCL-81 |
| A549 |
ATCC |
CCL-185 |
| Experimental Models: Organisms/Strains |
|
|
| C57BL/6J mice |
Jackson Laboratory |
000664 |
| hSTAT2 KI mice |
Diamond Laboratory |
NA |
| C1q−/− mice |
(Botto et al., 1998) |
NA |
| Oligonucleotides |
|
|
| ZIKV Dakar TaqMan primers |
(Gorman et al., 2018) |
NA |
| Recombinant DNA |
|
|
| pACgc46E1(d2t.L) |
Precigen |
NA |
| pGC46CMVTetO.MCS.SV |
Precigen |
NA |
| ZIKV prM-E |
Fremont Laboratory |
GenBank Accession # KJ776791.2
|
| Software and Algorithms |
|
|
| Prism |
Graphpad |
Version 8.1.1 |
| FlowJo |
FlowJo, LLC |
Version 10.0.7 |
