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. 2019 Sep 18;14(9):e0220208. doi: 10.1371/journal.pone.0220208

Table 1. Parameters for mass spectrometric detection of (glycated) amino acids after chromatographic separation.

target analyte precursor ion [m/z] product ion [m/z] dwell time [ms] fragmentor voltage [V] collision energy [eV]
FruLys (13C6,15N2) 317 233 70 70 13
FruLys (13C6,15N2) 317 129 70 70 15
FruLys 309 84 100 120 30
FruLys 309 225 100 120 10
Pyr (13C6,15N2) 263 182 100 75 5
Pyr (13C6,15N2) 263 153 100 75 20
Pyr 255 175 100 80 10
Pyr 255 148 100 80 20
MG-H1 (13C6) 235 74 100 75 20
MG-H1 (13C6) 235 115 100 75 10
MG-H1 229 114 100 120 10
MG-H1 229 166 100 120 10
CEL (13C3) 222 84 100 90 20
CEL (13C3) 222 130 100 90 10
CEL 219 130 100 100 10
CEL 219 84 100 100 20
CML (2H2) 207 84 100 90 20
CML (2H2) 207 130 100 90 10
CML 205 84 100 100 20
CML 205 130 100 100 10
Arg (13C6) 181 74 70 82 20
Arg (13C6) 181 61 70 82 10
Arg 175 70 70 90 20
Arg 175 60 70 90 10
Lys (13C6,15N2) 155 90 70 80 13
Lys (13C6,15N2) 155 137 70 80 3
Lys 147 84 70 75 10
Lys 147 130 70 75 10