Table 3. Parental alleles associated with increased/decreased trait values and significant mean differences observed in the RILs1 for phenotypic trait values when a particular parental allele is present at major QTL identified in this study.
| Trait/QTL | 2Allele for increased trait value | Trait value | Allele for decreased trait value | Trait value | t-test for mean difference (P-value) |
|---|---|---|---|---|---|
| Flour Extraction (%) | |||||
| QFE.ndsu.5B | AA | 58.1 | BB | 55.8 | 0.002556 |
| QFE.ndsu.3A.1 + QFE.ndsu.5A.2 | BB+BB | 62.6 | AA+AA | 59.2 | 0.000001 |
| QFE.ndsu.5B+ QFE.ndsu.3A.1 + QFE.ndsu.5A.2 | AA+BB+BB | 63.0 | BB+AA+AA | 58.9 | 0.000000 |
| Grain protein content (%) | |||||
| QGPC.ndsu.5B | BB | 15.2 | AA | 14.7 | 0.000493 |
| QGPC.ndsu.7A.2 | BB | 15.3 | AA | 14.7 | 0.000009 |
| QGPC.ndsu.7B | BB | 15.4 | AA | 14.7 | 0.000000 |
| QGPC.ndsu.7A.2 + QGPC.ndsu.7B | BB+BB | 15.9 | AA+AA | 14.5 | 0.000000 |
| QGPC.ndsu.5B + QGPC.ndsu.7A.2 + QGPC.ndsu.7B | BB+BB+BB | 16.2 | AA+AA+AA | 14.5 | 0.000001 |
| Grain Hardness | |||||
| QGH.ndsu.1A | BB | 72.2 | AA | 68.1 | 0.000000 |
| QGH.ndsu.1B | BB | 71.9 | AA | 68.6 | 0.000180 |
| QGH.ndsu.4B.1 | AA | 70.5 | BB | 69.1 | 0.054342 |
| QGH.ndsu.1A+QGH.ndsu.1B +QGH.ndsu.4B.1 | BB-BB-AA | 75.0 | AA-AA-BB | 60.7 | 0.000000 |
| Grains per spike | |||||
| QGPS.ndsu.2B | AA | 35.4 | BB | 33.5 | 0.000045 |
| QGPS.ndsu.3D | AA | 35.2 | BB | 33.8 | 0.002273 |
| QGPS.ndsu.2B + QGPS.ndsu.3D | AA+AA | 36.1 | BB+BB | 33.1 | 0.000007 |
| Heading date (no of days) | |||||
| QDH.ndsu.5A | BB | 57.9 | AA | 54.2 | 0.000000 |
| QDH.ndsu.5B | BB | 55.8 | AA | 55.1 | 0.069471 |
| QDH.ndsu.5A+ QDH.ndsu.5B | BB+BB | 56.7 | AA+AA | 52.5 | 0.000006 |
| Plant Height (cm) | |||||
| QPH.ndsu.2D | AA | 95.2 | BB | 86.3 | 0.000000 |
1 Only the RILs showing clear genotype for all the associated markers at the target QTL were used for the analysis
2AA = ND 705 allele; BB = PI 414566 allele