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. 2019 Sep 18;14(9):e0222336. doi: 10.1371/journal.pone.0222336

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© 2019 Funakoshi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) The expression of PADI3 and CDC42EP3 in breast cancer cell lines and macrophages. Expressions of PADI3 in SUM149 (TN-IBC), MDA-MB-231 (TN-non-IBC) cells, THP-1 (monocytes), M0 macrophages (THP-1-derived immature macrophages) and M2 macrophages (THP-1–derived M2 macrophages) were analyzed with Western blotting. Expressions of CDC42EP3 in SUM149 and MDA-MB231 were analyzed with Western blotting. (B) PADI3 knockdown suppresses cell growth in SUM149. Left panel: The expression of PADI3 in SUM149 cells was depleted with siRNA and the expression of PADI3 in siControl and siPADI3 was analyzed with western blot. Right panel: Proliferation of SUM149 cells transfected with siControl and siPADI3 was measured by Trypan blue exclusion assay. Bars, SD. (C) PADI3 knockdown suppresses anchorage-independent growth of SUM149 cells. Bars, SD.