Table 3. Primers used for the molecular cloning of the target genes.
Gene | Primer | Nucleotide sequence (5’- 3’) |
---|---|---|
Ocln | Forward | CATGGTGTGTGGATTCCTGGT |
Reverse | CGTCTCTTGCCCCTGTTGG | |
ZO-1 | Forward | GGCCATGAAACCGCAGTCAG |
Reverse | ATCTTTTCTCCACTGGGCTCAC | |
Cad-17 | Forward | AGAGGAGCTGGACAGAGA |
Reverse | CAGAGGCAGCTCATTGTTGA | |
E-Cad | Forward | TACACTGTGGTCCTGAGGGT |
Reverse | GTGCTGTCGGGTCATTGTCA | |
N-Cad | Forward | CGAACGCCATCAACATCAC |
Reverse | TAGACGGCGGATTCCCAC | |
α-Tub | Forward | AACTCCATCCTGACCACC |
Reverse | CCATCTGATTGGCTGGCTCA | |
Vim | Forward | TGCAGAGCTTCAGACAGGAT |
Reverse | GGCCATCTCGTCCTTCATGT |