Table 4. Primers used for the synthesis of standard mRNAs.
| Gene | Primer | Nucleotide sequence (5’- 3’) |
|---|---|---|
| Ocln | T7 Forward | taatacgactcactatagggTGGGTGAACAATGTGGAGGA |
| Reverse | CGTCTCTTGCCCCTGTTGG | |
| ZO-1 | T7 Forward | taatacgactcactatagggCCATGAAACCGCAGTCAG |
| Reverse | ACGGCGATCAAAGTAGGACA | |
| Cad-17 | T7 Forward | taatacgactcactatagggAGAGGAGCTGGACAGAGA |
| E-Cad | T3 Forward | caattaaccctcactaaaggg TACACTGTGGTCCTGAGGGT |
| Reverse | GTGCTGTCGGGTCATTGTCA | |
| Reverse | CAGAGGCAGCTCATTGTTGA | |
| N-Cad | T7 Forward | taatacgactcactatagggCGAACGCCATCAACATCAC |
| Reverse | TAGACGGCGGATTCCCAC | |
| α-Tub | T7 Forward | taatacgactcactatagggAACTCCATCCTGACCACC |
| Reverse | CCATCTGATTGGCTGGCTCA | |
| Vim | T7 Forward | taatacgactcactataggg TGCAGAGCTTCAGACAGGAT |
| Reverse | GGCCATCTCGTCCTTCATGT |