Table 5. Primers and probes used for one-step Taqman real-time RT-PCR.
| Gene | Primer | Nucleotide sequence (5’- 3’) |
|---|---|---|
| Ocln | Forward | GGACGAAGACGACAACAACGA |
| Reverse | CCATGGGAGAAAGCCTCTGA | |
| Taqman Probe | TACTCAGAGAGAACCACGAGCCGGCC | |
| ZO-1 | Forward | CGGCCTGCAGATGTTCCTAA |
| Reverse | GCTGAGGGAATTGGCTTTGA | |
| Taqman Probe | CCTGCGAGTGCACCTGGCCC | |
| Cad-17 | Forward | TGCCCACCTGACTTACATCATC |
| Reverse | TTCCAGTGGCAGCATCAATG | |
| Taqman Probe | CCGGATGATTCAGCAACCAAAACCTTCT | |
| E-Cad | Forward | CGGAGAGGATGATCAGGACTATG |
| Reverse | TACTGTGGAGCTGGCATGAAGT | |
| Taqman Probe | TTCACCGTGGTCTGGACAACCGA | |
| N-Cad | Forward | CGTGCTGCTGTTTGTGGTATG |
| Reverse | CTCACATCATCCTCTGGATCGA | |
| Taqman Probe | AAGAACGTCAGGCGAAGCAGCTCCTC | |
| α-Tub | Forward | AGGCTCATTGGCCAGATTGT |
| Reverse | CAACATTCAGGGCTCCATCA | |
| Taqman Probe | TCTTCAATCACAGCCTCGCTTCGCT | |
| Vim | Forward | GATGTCCGCCTGCAGTATGA |
| Reverse | GGTGAGGTCAGCAAACTTGGA | |
| Taqman Probe | AACCTGGCCTCCAAAAACATCCATGAG |