KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti c-fos (1:3,000) | Santa Cruz Biotechnology | sc-52 |
| Mouse anti-PKC-δ (1:500) | BD Biosciences | 610398 |
| Rabbit Somatostatin-14 (1:2,000) | Peninsula Laboratories | T-4103 |
| Mouse anti-TH (1:1,000) | Immunostar | 22941 |
| Goat anti CGRP (1:500) | Abcam | ab36001 |
| Rabbit anti-RFP | Rockland Immunochemicals | 600–401-379 |
| Bacterial and Virus Strains | ||
| AAV8-hSyn-DIO-hM4d-mCherry | (Krashes et al., 2011) | UNC Vector Core AV4908B/Addgene 44362 |
| AAV8-hSyn-DIO-mCherry | (Krashes et al., 2011) | UNC Vector Core 4981CD |
| AAV5-EF1a-DIO-ChR2-eYFP | Deisseroth Lab | UNC Vector Core |
| AAV5-EF1a-DIO-taCasp3-tevP | (Yang et al., 2013) | UNC Vector Core |
| AAV8.2-EF1a-DIO-Synaptophysin-mCherry | (Opland et al., 2013) | MIT Vector Core |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Cholera Toxin Subunit B 555 | Thermofisher Scientific | C34776 |
| CNO | Gift from Bryan Roth and Hello Bio | HB6149 |
| Critical Commercial Assays | ||
| Tyramide Signal Amplification Kit | Perkin Elmer | NEL701001KT |
| viewRNA Probes | Affymetrix (now Thermofisher) | NA |
| Mm-Pnoc | Affymetrix (now Thermofisher) | VB1–3030997-VT |
| Cre | Affymetrix (now Thermofisher) | VF1–13057 |
| RNAscope Probes | Advanced Cell Diagnostics | NA |
| Mm-Pnoc | Advanced Cell Diagnostics | 437881 |
| Mm-Crh | Advanced Cell Diagnostics | 316091-C2 |
| Mm-Nts | Advanced Cell Diagnostics | 420441-C2 |
| Mm-Tac2 | Advanced Cell Diagnostics | 446391-C2 |
| Mm-Calcr | Advanced Cell Diagnostics | 452281-C2 |
| Mm-Htr2a | Advanced Cell Diagnostics | 401291-C2 |
| Mm-Prkcd | Advanced Cell Diagnostics | 441791-C2 |
| Mm-Sst | Advanced Cell Diagnostics | 404631-C2 |
| Experimental Models: Organisms/Strains | ||
| Pnoc- IRES-Cre | This paper | N/A |
| C57BL6/J | The Jackson Laboratory | Stock #000664 |
| B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | The Jackson Laboratory | Stock #007909 |
| B6.Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10)Dolsn | Kind gift from Brad Lowell | MGI: 5559562 |
| Software and Algorithms | ||
| Zen Blue Advanced Processing Module | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen/image-analysis.html |
| pClamp/Clampfit 10.7 | Molecular Devices | http://mdc.custhelp.com/app/answers/detail/a_id/18779/~/axon™pclamp™−10-electrophysiology-data-acquisition-%26-analysis-software-download |
| Image processing, cell counter module | ImageJ/Fiji | https://fiji.sc/ |
| Prism 6.0h | Graphpad | https://www.graphpad.com/ |
| Imaris | Bitplane | https://www.bitplane.com/imaris |
| Clearmap | (Renier et al., 2016) | https://github.com/ChristophKirst/ClearMap |
| Omniplex Neural Data Acquisition System/Offline Sorter | Plexon | https://plexon.com/products/omniplex-d-neural-data-acquisition-system-1/ |