Fig. 7. ID3 is required for development of ID2-deficient NK cells.

(A) Accessible chromatin tracks for the indicated populations surrounding the Id3 gene. Arrows indicate regions that are more accessible in RId2^−/− as compared to Ctrl NK cells. The red arrows indicate peaks unique to NK cells. (B) CD45.2⁺ CD3ε⁻TCRβ⁻TCRδ⁻NK1.1⁺DX5⁺ NK cell numbers/10⁴ BM cells in chimeric mice created by transplanting Id3^+/+ (black) or Id3^−/− (grey) bone marrrow into lethally irradiated CD45.1⁺ mice. Each circle represents one transplanted mouse with a unique donor. N = 4. (C) Flow cytometry of BM for CD45.2⁺ NK cells in CD54.1⁺ mice transplanted with CD45.2⁺ Ctrl, LId2^−/− and LId2^−/−Id3^−/− BM. Each row of plots is gated on the population indicated in the row to the left. Lineage = CD3ε, TCRβ, TCRγ. (D) Average percent reconstitution, determined as the percent of Lin⁻CD122⁺NK1.1⁺DX5⁺ cells that were CD45.2⁺ + S.D^. is shown for each population. N= 5. (E) and (F) are for the spleen as in (C) and (D). ** P<0.01, *** P<0.001.