Figure 2. Analysis of Tet2 Mut and KO Mouse Bone Marrow.

(A) Percentages of the indicated fractions in the bone marrow of Tet2 Mut and KO mice (4 months old) (n = 6).

(B) Bone marrow flow analysis for myeloid and lymphoid cells from Tet2 Mut and KO mice (10 months old).

(C and D) Representative flow data for myeloid progenitors and HSPCs in the bone marrow from Tet2 Mut and KO mice (10 months old). Percentages of the indicated fractions (percentage in Lin⁻Sca1⁻c-Kit⁺ cells for CMPs, GMPs, and MEPs; percentage in Lin⁻CD127⁺ cells for CLPs; percentage in Lin⁻ cells for LSK; and percentage in CD135⁻LSK cells for CD150⁺ HSCs) (n = 6).

(E) Tet2-KO HSPCs have increased replating capacity compared to Tet2-Mut HSPCs (10 months old, up to fourth -round replating data) (n = 3).

(F) Representative images of May-Grünwald-Giemsa-stained cytospin preparations of the colonies formed by HSPCs from Tet2 WT, Mut, and KO mice (at second replating). Metamyelocytes, myelocytes, and matured granulocytes consist of WT colonies, and immature and dysplastic myeloid cells are increased in Tet2 Mut and KO colonies (also see Figure S2H). Scale bars, 10 μm.

HSPCs, hematopoietic stem and progenitor cells; LSK, Lin⁻c-Kit⁺Sca-1⁺; MEP, megakaryocyte-erythrocyte progenitor, Lin⁻Sca-1⁻c-Kit⁺CD16/32^low/negCD34^low/neg; GMP, granulocyte-monocyte progenitor, Lin⁻Sca-1⁻c-Kit⁺CD16/32^highCD34⁺; CMP, common myeloid progenitor, Lin⁻Sca-1⁻c-Kit⁺CD16/32^lowCD34⁺; CLP, common lymphoid progenitor, Lin⁻IL7Rα⁺c-Kit^midSca-1⁺. Error bars indicate SD. n.c. stands for no significant change. See also Figure S2.