| Antibodies |
|
|
| Anti-Tet2 |
Abcam |
Cat# ab124297; RRID: AB_2722695 |
| Anti-Actin |
Abcam |
Cat# AC-15; RRID: AB_1658432 |
| Anti-5hmC |
Active Motif |
Cat# 39770; RRID: AB_10013602 |
| Goat Anti-Rabbit IgG-HRP |
CalBiochem |
Cat# 401393–2ML; RRID: AB_437797 |
| Goat Anti-Mouse IgG-HRP |
CalBiochem |
Cat# 401253; RRID: AB_437779 |
| CD4 (GK1.5) |
BD Bioscience |
Cat# 553728; RRID: AB_395012 |
| B220 (RA3–6B2) |
BD Bioscience |
Cat# 553086; RRID: AB_394616 |
| TER-119 (TER-119) |
BD Bioscience |
Cat# 553672; RRID: AB_394985 |
| CD11b (M1/70) |
BD Bioscience |
Cat# 553309; RRID: AB_394773 |
| CD19 (1D3) |
BD Bioscience |
Cat# 553784; RRID: AB_395048 |
| CD43 (S7) |
BD Bioscience |
Cat# 560663; RRID: AB_1727479 |
| NK-1.1 (PK136) |
BD Bioscience |
Cat# 553163; RRID: AB_394675 |
| c-Kit (2B8) |
BioLegend |
Cat# 105826; RRID: AB_1626278 |
| Gr-1 (RB6–8C5) |
BioLegend |
Cat# 108404; RRID: AB_313369 |
| CD135 (A2F10) |
eBiosciences |
Cat# 12–1351–82; RRID: AB_465859 |
| CD34 (RAM34) |
eBiosciences |
Cat# 11–0341–85; RRID: AB_465022 |
| Sca-1 (D7) |
eBiosciences |
Cat# 25–5981–81; RRID: AB_469668 |
| CD3e (145–2C11) |
eBiosciences |
Cat# 13–0031–85; RRID: AB_466320 |
| CD8 (53–6.72) |
eBiosciences |
Cat# 13–0081–85; RRID: AB_466347 |
| IgM (II/41) |
eBiosciences |
Cat# 13–5790–85; RRID: AB_466676 |
| CD71 (R17217) |
eBiosciences |
Cat# 11–0711–82; RRID: AB_465124 |
| CD127 (A7R34) |
eBiosciences |
Cat# 12–1271–82; RRID: AB_465844 |
| CD45.2 or Ly5.2 (104) |
eBiosciences |
Cat# 11–0454–82; RRID: AB_465061 |
| CD45.1 or Ly5.1 (A20) |
eBiosciences |
Cat# 25–0453–81; RRID: AB_469628 |
| Streptavidin-APC-eFluor780 |
eBiosciences |
Cat# 47–4317–82; RRID: AB_10366688 |
| Streptavidin-PerCP-Cy5.5 |
eBiosciences |
Cat# 45–4317–82; RRID: AB_10311495 |
| Critical Commercial Assays |
|
|
| RNAeasy Mini kit |
QIAGEN |
74104 |
| Superscript III first strand |
Invitrogen |
18080–400 |
| XtremeGene 9 DNA transfection reagent |
Roche |
06365787001 |
| Deposited Data |
|
|
| RNA-seq data |
This paper |
GEO: GSE132090
|
| Experimental Models: Organisms/Strains |
|
|
|
Tet2−/− (Tet2 KO) mouse |
Li et al., 2011 |
N.A. |
|
Tet2m/m (Tet2 Mut) mouse |
This paper |
N.A. |
| B6.SJL-Ptprca Pepcb/BoyJ |
Jackson lab |
002014 |
| Oligonucleotides |
|
|
| RT-qPCR primers, see Table S1
|
This paper |
n/a |
| Genotyping Primers, see Table S1
|
This paper |
n/a |
| gRNA oligos for gene targeting, see Table S1
|
This paper |
n/a |
| Recombinant DNA |
|
|
| Topo pcr2.1 donor vector |
This paper |
n/a |
| pX330-Tet2-gRNA |
This paper |
n/a |
| FUW-ires-puro |
This paper |
n/a |
| FUW-HA-mGata2-ires-puro |
This paper |
n/a |
| Software and Algorithms |
|
|
| Trim galore v0.4.1 |
Github |
https://github.com/FelixKrueger/TrimGalore |
| Tophat v2.0.13 |
John Hopkins University |
https://ccb.jhu.edu/software/tophat/index.shtml |
| HTSeq v0.6.1 |
Anders et al., 2015 |
https://github.com/simon-anders/htseq |
| Cufflinks v2.2 |
Trapnell et al., 2010 |
http://cole-trapnell-lab.github.io/cufflinks/ |
| DESeq2 v1.20.0 |
Love et al., 2014 |
http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html |
| DAVID 6.8 |
Jiao et al., 2012 |
https://david.ncifcrf.gov/ |
| GraphPad Prism 7 |
GraphPad |
https://www.graphpad.com/ |
| ImageJ |
Schneider et al., 2012 |
https://imagej.nih.gov/ij/ |
