Table 1.
Animal Characteristics
| WT |
KO |
|||
|---|---|---|---|---|
| Control | IL-6 | Control | IL-6 | |
| n | 14 | 15 | 12 | 11 |
| Body Weights (g) | ||||
| Pre | 23.3 ± 1.5 | 23.4 ± 1.9 | 24.7 ± 2.0 | 24.0 ± 1.7 |
| Mid | 24.0 ± 1.5 | 23.4 ± 1.4 | 24.9 ± 2.0 | 24.1 ± 1.3 |
| Post | 24.9 ± 2.0 | 24.0 ± 2.1 | 25.1 ± 1.3 | 24.3 ± 1.7 |
| Plasma IL-6 (pg/mL) | ||||
| Pre | - | - | - | - |
| Mid | - | 63 ± 52* | - | 98 ± 55& |
| Post | - | 75 ± 43* | - | 141 ± 31& |
| Tissues at Sacrifice | ||||
| Hindlimb (mg) | 207 ± 14 | 200 ± 14 | 214 ± 11@ | 206 ± 14@ |
| Hindlimb/Tibia (mg/mm) |
12.2 ± 0.7 | 12.0 ± 0.7 | 12.7 ± 0.6 | 12.3 ± 0.8 |
| Tibia Length (mm) | 17.0 ± 0.3 | 16.8 ± 0.2# | 16.8 ± 0.2 | 16.7 ± 0.2# |
| Epididymal Fat (mg) | 362 ± 139 | 313 ± 96 | 308 ± 52 | 290 ± 85 |
| Spleen (mg) | 71 ± 12 | 122 ± 26# | 93 ± 68 | 199 ± 92# |
Values are means ± SD. Male C57BL/6 (WT) mice. Male skeletal muscle specific gp130 knockout (KO) mice. Age in weeks (wks). Body weight in grams (g). Plasma interleukin-6 (IL-6) in picograms/milliliters (pg/mL). All vector controls and pre-IL-6 levels fell below the detection limit and therefore weren’t reported. Combined weight of the soleus, plantaris, gastrocnemius, and tibialis anterior (hindlimb) in milligrams (mg). Tibia length in millimeters (mm). Epididymal fat pad weight in milligrams. Spleen weight in milligrams. Significance set at p<0.05 (Two-Way ANOVA).
Significant main effect of IL-6.
Significant main effect of KO. & Significantly different from all groups.
WT-IL-6 significant interaction from all groups.
KO-IL-6 significant interaction from all groups.