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. Author manuscript; available in PMC: 2020 Sep 5.
Published in final edited form as: Cell. 2019 Sep 5;178(6):1313–1328.e13. doi: 10.1016/j.cell.2019.08.010

Figure 3. Transcriptional Responses to TIV in Control and Antibiotics-Treated Subjects.

Figure 3.

(A) Number of genes differentially expressed (log2 fold-change > 0.2 and t-test p value < 0.01) relative to day 0 in control and antibiotics-treated subjects on days 1, 3, and 7 post-vaccination.

(B) BTMs significantly enriched (FDR < 0.05, NES ≥ 2) in control and antibiotics-treated subjects post-vaccination. GSEA (Subramanian et al., 2005) was used to identify positive (red) or negative (green) enrichment of BTMs using ranked gene lists, where genes were ordered by t-statistic based on post-vaccination fold change relative to day 0.

(C-D) Temporal expression patterns of genes within modules M127 and M156.1 among antibiotics-treated (red) and control subjects (blue). Black line represents the mean fold change of all genes.

See also Figure S4 for comparison of responses in phases 1 and 2.