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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Cell Rep. 2019 Sep 3;28(10):2689–2703.e4. doi: 10.1016/j.celrep.2019.07.103

Figure 6. Oct4 Negatively Influences Sox2 Binding to the Nucleosome Dyad.

Figure 6.

(A) Diagram of nucleosome substrates containing a dyad-positioned Sox2:Oct4 composite motif, either with no gap (NucSO-Dyad) or with a 3 bp gap between the Sox2 and Oct4 motifs (NucSO+3-Dyad).

(B) Average residence times (ton) of Sox2 on different DNA and nucleosome substrates shown in (A) in the absence or presence of Oct4.

(C) Time constants for the specific Sox2 binding mode (τ2) on NucSO-Dyad and NucSO+3-Dyad in the absence or presence of Oct4.

(D) Relative populations of specific Sox2 binding events (A2) in the absence or presence of Oct4.

(E) A representative EMSA gel showing the formation of Sox2-NucSO-Dyad complexes at different Sox2 concentrations in the absence or presence of Oct4.

(F) Dissociation constant (KD) for the Sox2-NucSO-Dyad interaction in the absence or presence of Oct4 determined from the EMSA results. n = 3 experimental replicates.

(G) Average residence times (ton) of Oct4 on different DNA and nucleosome substrates containing a dyad-positioned composite motif in the absence or presence of Sox2.

Data are represented as mean ± SD. See also Figures S5 and S6.