Table 2. Contraction-Relaxation Durations and Beating Frequencies of the hiPSC-Derived Cardiomyocyte Aggregates Under All Tested Conditions, Analyzed with BeatView Software; n = 4.
| material | 2D/3D | ratioc [mg mL –1] | beating rate [BPM] | standard deviation | contraction–relaxation duration [ms] | standard deviation |
|---|---|---|---|---|---|---|
| gelatin coating control | 2D | 100:0 | 35.78 | ±20.41 | 568.60 | ±127.10a |
| GELA-ADH coating | 100:0 | 42.35 | ±6.69 | 435.50 | ±154.02 | |
| GELA-CDH coating | 100:0 | 35.60 | ±20.18 | 662.44 | ±268.50a | |
| F7-SPD | 3D | 0:100 | 0 | 0 | ||
| F1–3-ADH | 2D | 40:40 | 36.71 | ±17.74 | 435.25 | ±113.70 |
| 40:20 | 68.04 | ±17.01 | 305.21 | ±65.13 | ||
| 3D | 40:40 | 72.10 | ±15.14 | 264.14 | ±41.97 | |
| 40:20 | 52.70 | ±47.60 | 474.59 | ±303.62a | ||
| F4–6-CDH | 2D | 60:60 | 38.63 | b | 423.74 | b |
| 60:40 | 37.73 | ±2.78b | 434.34 | ±38.96b | ||
| 40:40 | 41.56 | ±5.33 | 393.14 | ±63.67 | ||
| 3D | 60:60 | 35.77 | ±7.00 | 403.68 | ±44.01 | |
| 60:40 | 37.99 | ±6.50 | 452.44 | ±32.85 | ||
| 40:40 | 33.82 | ±3.02 | 491.88 | ±15.65 |
a
Major prolongation in contraction–relaxation interval detected in one sample.
b
Elasticity of hydrogel transferring movement over a long distance interferes with the beating analysis; so only one or two aggregates were analyzed successfully.
c
Ratio of gelatin to GG.