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. 2019 Sep 13;12:7501–7512. doi: 10.2147/OTT.S209138

Figure 3.

Figure 3

ZFAS1 negatively regulated miR-590-3p.

Notes: (A) Bioinformatics analysis showed the prediction for miR-590-3p binding sites on ZFAS1. (B) Luciferase activity in HEK 293T cells co-transfected with miR-590-3p mimics or miR-NC and luciferase reporters containing wild type ZFAS1 or mutant ZFAS1. (C) Relative expression of ZFAS1 in K-1 cells transfected with si-ZFAS1 or si-NC and in TPC-1 cells transfected with pcDNA-ZFAS1 or Empty vetor. (D) Relative expression levels of miR-590-3p in 80 pairs of PTC tissues and adjacent noncancerous tissues. (E) The correlation between ZFAS1 and miR-590-3p expression were measured in 80 PTC tissues by Pearson correlation analysis. (r=−0.5380, P<0.0001). (F) Relative expression levels of miR-590-3p in human thyroid follicular epithelial cell line Nthy-ori 3-1 and four PTC cell lines (B-CPAP, IHH-4, TPC-1 and K-1). (G) Association of ZFAS1 and miR-590-3p with Ago2 in K-1 cells. (H) Association of ZFAS1 and miR-590-3p with Ago2 in TPC-1 cells. *P<0.05, **P<0.01.

Abbreviations: WT, wild‑type; MUT, mutant, NC, negative control.