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. 2019 Sep 13;12:7501–7512. doi: 10.2147/OTT.S209138

Figure 4.

Figure 4

ZFAS1 promoted cell proliferation and inhibited apotosis by regulating miR-590-3p.

Notes: (A) CCK-8 assay was used to determine the cell viability by transfecting si-NC, si-ZFAS1, miR-590-3p inhibitor or si-ZFAS1+ miR-590-3p inhibitor into K-1 cell. (B) CCK-8 assay was used to determine the cell viability by transfecting Empty vetor, pcDNA-ZFAS1, miR-590-3p mimics or pcDNA-ZFAS1+ miR-590-3p mimics into TPC-1 cell. (C, D) Similarly, cell viability also was detected by the Edu assay in K-1 cells and TPC-1 cells. (E) Apoptosis of K-1 cells cotransfected with si-NC, si-ZFAS1, miR-590-3p inhibitor or si-ZFAS1+ miR-590-3p, and apoptosis of TPC-1 cells cotransfected with Empty vetor, pcDNA-ZFAS1, miR-590-3p mimics or pcDNA-ZFAS1+ miR-590-3p mimics. (F) Effect of si-NC, si-ZFAS1, miR-590-3p inhibitor or si-ZFAS1+ miR-590-3p on BAX and BCL-2 protein levels in K-1 cells. (G) Effect of Empty vetor, pcDNA-ZFAS1, miR-590-3p mimics or pcDNA-ZFAS1+ miR-590-3p mimics on BAX and BCL-2 protein levels in TPC-1 cells. *P<0.05, **P<0.01.

Abbreviation: NC, negative control.