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. 2019 Sep 13;12:7501–7512. doi: 10.2147/OTT.S209138

Figure 5.

Figure 5

ZFAS1 promotes HMGA2 expression by regulating miR-590-3p.

Notes: (A) The candidate binding sites of miR-590-3p in HMGA2 mRNA 3ʹ-UTR. (B) Luciferase activity in HEK 293T cells co-transfected with miR-590-3p mimics or miR-NC and luciferase reporters containing wild type HMGA2 or mutant HMGA2. (C) The relative mRNA expression of HMGA2 was detected by transfecting with Empty vetor, pcDNA-ZFAS1, miR-590-3p mimics or pcDNA-ZFAS1+ miR-590-3p mimics in TPC-1 cells. (D) The relative mRNA expression of HMGA2 was detected by transfecting with si-NC, si-ZFAS1, miR-590-3p inhibitor, or si-ZFAS1+ miR-590-3p inhibitor in K-1 cells. (E) Relative expression levels of HMGA2 in 80 pairs of PTC tissues and adjacent noncancerous tissues. (F) The correlation between HMGA2 and miR-590-3p expression were measured in 80 PTC tissues by Pearson correlation analysis. (r = −0.5422, P<0.0001). (G) The correlation between ZFAS1 and HMGA2 expression were measured in 80 PTC tissues by Pearson correlation analysis. (r =0.6093, P<0.0001). (H, I) The relative protein expression of HMGA2 was analyzed by transfecting with transfecting with si- NC, si-ZFAS1, miR- 590-3p inhibitor, or si-ZFAS1+ miR-590-3p inhibitor into K-1 cells or transfecting with Empty vetor, pcDNA- ZFAS1, miR-590-3p mimics, or pcDNA-ZFAS1+ miR-590-3p mimics into TPC-1 cells. *P<0.05, **P<0.01, ****P<0.0001.

Abbreviations: NC, negative control, PTC, papillary thyroid carcinoma.