Figure 5.

iSCAT tracking on the live cell. (a) Three-dimensional trajectory of a vaccinia virus interacting with a live HeLa cell surface. The virus is seen to undergo immediate nanoscale confinement. Trajectory recorded at 5000 fps. Adapted from ref (31). (b) A long trajectory of 75 000 data points recorded from a GNP-labeled transmembrane protein on a live HeLa cell, tracked to few-nanometer precision. (c) Temporal accumulated residency of the protein confirms regions of prolonged confinement. (d) Three-dimensional interpolated surface of a trajectory showing nanoscale confinement of the protein upon the membrane surface, consistent with confinement into a clathrin pit preceding endocytosis. (b–d) Adapted from ref (20).