Figure 5.

Constitutively active αvβ3 signaling does not alter α5β1 integrin localization within fibrillar adhesions. Subconfluent EV, WTβ3, or CAβ3 cells were plated onto glass coverslips for 24 hours prior to fixation. Cells were double-labeled with mAb SNAKA51 against the active α5β1 integrin (D, G, J) and rabbit anti-fibronectin antiserum (FN) (E, H, K). As a control, EV cells (A–C) were double-labeled with mAb GAL-13 against β-galactosidase (A) and R-NIS (B). Extensive colocalization (arrowheads) of α5β1 integrin and fibronectin within fibrillar adhesions was observed in all three cell lines (F, I, L). WTβ3 and CAβ3 cells (not shown) were also double-labeled with mAb GAL-13 and R-NIS with identical results as observed with EV cells. This labeling was performed three times with identical results. Scale bar: 50 μm.