Figure 7.

CAβ3 cells demonstrate elevated RhoA activity in response to serum. EV and CAβ3 cells were serum starved for ∼16 hours prior to simulation with 10% FBS for 15 or 30 minutes prior to G-LISA analysis. EV cells failed to demonstrate statistically significant RhoA activation under these conditions. CAβ3 cells responded to serum after 30 minutes with a significant increase in RhoA activity (P < 0.02) over untreated cells or cells treated for 15 minutes with serum (P < 0.045). Results are pooled from three separate assays with triplicate determinations (n = 9) and represent the mean ± SE.