Figure 3.

Comparison of the relative enzymatic activities of DnrK, RdmB, ZamB, EamK, TamK, and CalMB. The rows depict substrates 2, 4, and 5 used in reaction with the 15-demethylases (EamC and DnrP) and subsequent reactions of the various SAM-dependent methyltransferase-like enzymes. The columns present the formation of 10-decarboxylation, 4-O-methylation, and 10-hydroxylation reaction products with the non-, mono-, and triglycosylated substrates. The overall percentages may not add up to 100% in all samples due to remaining unreacted substrate with proteins harboring poor activity. Legend: R = −OH or l-rhodosamine or l-rhodosamine-2-deoxyfucose-cinerulose A.