Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli EcNR2) |
MG1655, bla, bio-, λ-Red+, mutS-::cmR |
PMID: 19633652 | Addgene #26931 | |
| Sequence-based reagent |
90 bp DNA oligos with phosphorothioated bases |
This paper | See Supplementary file 4 | 100 nmole DNA Plate oligo, Integrated DNA Technologies |
| Commercial assay or kit | Miseq Reagent Kit V2 | Illumina | MS-102–2002 | |
| Commercial assay or kit | sybr green | ThermoFisher | S7567 | |
| Commercial assay or kit | Qubit HS DNA kit | ThermoFisher | Q32854 | |
| Commercial assay or kit | Q5 Hot Start High- Fidelity Mastermix |
NEB | M0494S | |
| Commercial assay or kit | DNA clean and concentration kit 5 |
Zymo Research | D4013 | |
| Commercial assay or kit | illustra bacteria genomicPrep Mini Spin kit |
GE life sciences | 28904259 | |
| Commercial assay or kit | Agilent DNA 1000 kit | Agilent Genomics | 5067–1504 | |
| Software, algorithm | SeqPrep v1.1 | John St. John | https://github.com/jstjohn/SeqPrep | |
| Software, algorithm | Bowtie2 | PMID: 22388286 | ||
| Software, algorithm | Perl scripts to count mutant reads |
This paper |
https://github.com/platyias/count-MAGE-seq
(copy archived at https://github.com/elifesciences-publications/count-MAGE-seq). |
|
| Other | Turbidostat for growth competition assay |
PMID: 23429717 |